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Extracellular Staining of Surface Markers RAW 264.7 cells (2 × 10 5 ) were infected with Ms_Vc or Ms_Rv1954A in incomplete DMEM for 4 h at MOI of 1:10 at 37°C followed by washing thrice with PBS and kept in complete medium containing gentamicin to kill extracellular bacteria for 48 h

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